rabbit polyclonal anti-cx37 antibody (Millipore)
Structured Review

Rabbit Polyclonal Anti Cx37 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti-cx37+antibody/rabbit+antibodies+to+cx37/pmc08928848-55-47-61
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α"
Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
Journal: Animal Cells and Systems
doi: 10.1080/19768354.2021.2023037
Figure Legend Snippet: List of oligonucleotide primers used for real-time PCR analysis.
Techniques Used: Real-time Polymerase Chain Reaction, Sequencing
Figure Legend Snippet: Immunofluorescence results showing effect of simvastatin on GJIC targeting Cx37, Cx40 and Cx43. Cx37 (A) and Cx40 (B) expression was downregulated in gap junctions of HUVECs treated with TNF-α relative to the control group. Treatment of HUVECs with simvastatin upregulated Cx37 and Cx40 expression. Similarly, HUVECs treated with TNF-α exhibited Cx43 up-regulation (C) although simvastatin reversed this phenomenon. D: Quantitative comparison of FITC staining intensity normalized to control. n = 6, * P < 0.05, ** P < 0.01 vs. Control; # P < 0.05, ## P < 0.01 vs. TNF-α (+) Simvastain (−) group. Bar = 30 μm.
Techniques Used: Immunofluorescence, Expressing, Staining
Figure Legend Snippet: Differential expression of Cx37, Cx40 and Cx43 mRNAs in HUVECs across groups. Cx37 and Cx40 had a 2-fold downregulation, whereas Cx43 was upregulated relative to the control group. Cx37 and Cx40 were significantly upregulated in HUVECs treated with TNF-α in combination with simvastatin relative to those treated with TNF-α alone. Cx43 was significantly downregulated. Data presented are means ± SEM, n = 6, ** P < 0.01 versus only TNF-α treatment group.
Techniques Used: Expressing
Figure Legend Snippet: Cx37, Cx40 and Cx43 proteins were differentially expressed in HUVECs in different treatment groups. A: Western blots. 1: Control, 2: TNF-α (+)/Simvastatin (−), 3: TNF-α (+)/Simvastatin (+). B: qRT-PCR results showing relative transcript levels. Data presented are means ± SEM, n = 6, ** P < 0.01 versus only TNF-α treatment group.
Techniques Used: Western Blot, Quantitative RT-PCR
Related Articles
Blocking Assay:Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and Molecular Weight:Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and Incubation:Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and Real-time Polymerase Chain Reaction:Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and Sequencing:Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and Immunofluorescence:Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and Expressing:Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and Staining:Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and Western Blot:Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and Quantitative RT-PCR:Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and |