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rabbit polyclonal anti-cx37 antibody  (Millipore)


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    Structured Review

    Millipore rabbit polyclonal anti-cx37 antibody
    List of oligonucleotide primers used for real-time PCR analysis.
    Rabbit Polyclonal Anti Cx37 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti-cx37+antibody/rabbit+antibodies+to+cx37/pmc08928848-55-47-61
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-cx37 antibody - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α"

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α

    Journal: Animal Cells and Systems

    doi: 10.1080/19768354.2021.2023037

    List of oligonucleotide primers used for real-time PCR analysis.
    Figure Legend Snippet: List of oligonucleotide primers used for real-time PCR analysis.

    Techniques Used: Real-time Polymerase Chain Reaction, Sequencing

    Immunofluorescence results showing effect of simvastatin on GJIC targeting Cx37, Cx40 and Cx43. Cx37 (A) and Cx40 (B) expression was downregulated in gap junctions of HUVECs treated with TNF-α relative to the control group. Treatment of HUVECs with simvastatin upregulated Cx37 and Cx40 expression. Similarly, HUVECs treated with TNF-α exhibited Cx43 up-regulation (C) although simvastatin reversed this phenomenon. D: Quantitative comparison of FITC staining intensity normalized to control. n = 6, * P < 0.05, ** P < 0.01 vs. Control; # P < 0.05, ## P < 0.01 vs. TNF-α (+) Simvastain (−) group. Bar = 30 μm.
    Figure Legend Snippet: Immunofluorescence results showing effect of simvastatin on GJIC targeting Cx37, Cx40 and Cx43. Cx37 (A) and Cx40 (B) expression was downregulated in gap junctions of HUVECs treated with TNF-α relative to the control group. Treatment of HUVECs with simvastatin upregulated Cx37 and Cx40 expression. Similarly, HUVECs treated with TNF-α exhibited Cx43 up-regulation (C) although simvastatin reversed this phenomenon. D: Quantitative comparison of FITC staining intensity normalized to control. n = 6, * P < 0.05, ** P < 0.01 vs. Control; # P < 0.05, ## P < 0.01 vs. TNF-α (+) Simvastain (−) group. Bar = 30 μm.

    Techniques Used: Immunofluorescence, Expressing, Staining

    Differential expression of Cx37, Cx40 and Cx43 mRNAs in HUVECs across groups. Cx37 and Cx40 had a 2-fold downregulation, whereas Cx43 was upregulated relative to the control group. Cx37 and Cx40 were significantly upregulated in HUVECs treated with TNF-α in combination with simvastatin relative to those treated with TNF-α alone. Cx43 was significantly downregulated. Data presented are means ± SEM, n = 6, ** P < 0.01 versus only TNF-α treatment group.
    Figure Legend Snippet: Differential expression of Cx37, Cx40 and Cx43 mRNAs in HUVECs across groups. Cx37 and Cx40 had a 2-fold downregulation, whereas Cx43 was upregulated relative to the control group. Cx37 and Cx40 were significantly upregulated in HUVECs treated with TNF-α in combination with simvastatin relative to those treated with TNF-α alone. Cx43 was significantly downregulated. Data presented are means ± SEM, n = 6, ** P < 0.01 versus only TNF-α treatment group.

    Techniques Used: Expressing

    Cx37, Cx40 and Cx43 proteins were differentially expressed in HUVECs in different treatment groups. A: Western blots. 1: Control, 2: TNF-α (+)/Simvastatin (−), 3: TNF-α (+)/Simvastatin (+). B: qRT-PCR results showing relative transcript levels. Data presented are means ± SEM, n = 6, ** P < 0.01 versus only TNF-α treatment group.
    Figure Legend Snippet: Cx37, Cx40 and Cx43 proteins were differentially expressed in HUVECs in different treatment groups. A: Western blots. 1: Control, 2: TNF-α (+)/Simvastatin (−), 3: TNF-α (+)/Simvastatin (+). B: qRT-PCR results showing relative transcript levels. Data presented are means ± SEM, n = 6, ** P < 0.01 versus only TNF-α treatment group.

    Techniques Used: Western Blot, Quantitative RT-PCR

    Related Articles

    Blocking Assay:

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Molecular Weight:

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Incubation:

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Real-time Polymerase Chain Reaction:

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Sequencing:

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Immunofluorescence:

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Expressing:

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Staining:

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Western Blot:

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Quantitative RT-PCR:

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α
    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).



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    Image Search Results


    List of oligonucleotide primers used for real-time PCR analysis.

    Journal: Animal Cells and Systems

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α

    doi: 10.1080/19768354.2021.2023037

    Figure Lengend Snippet: List of oligonucleotide primers used for real-time PCR analysis.

    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Techniques: Real-time Polymerase Chain Reaction, Sequencing

    Immunofluorescence results showing effect of simvastatin on GJIC targeting Cx37, Cx40 and Cx43. Cx37 (A) and Cx40 (B) expression was downregulated in gap junctions of HUVECs treated with TNF-α relative to the control group. Treatment of HUVECs with simvastatin upregulated Cx37 and Cx40 expression. Similarly, HUVECs treated with TNF-α exhibited Cx43 up-regulation (C) although simvastatin reversed this phenomenon. D: Quantitative comparison of FITC staining intensity normalized to control. n = 6, * P < 0.05, ** P < 0.01 vs. Control; # P < 0.05, ## P < 0.01 vs. TNF-α (+) Simvastain (−) group. Bar = 30 μm.

    Journal: Animal Cells and Systems

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α

    doi: 10.1080/19768354.2021.2023037

    Figure Lengend Snippet: Immunofluorescence results showing effect of simvastatin on GJIC targeting Cx37, Cx40 and Cx43. Cx37 (A) and Cx40 (B) expression was downregulated in gap junctions of HUVECs treated with TNF-α relative to the control group. Treatment of HUVECs with simvastatin upregulated Cx37 and Cx40 expression. Similarly, HUVECs treated with TNF-α exhibited Cx43 up-regulation (C) although simvastatin reversed this phenomenon. D: Quantitative comparison of FITC staining intensity normalized to control. n = 6, * P < 0.05, ** P < 0.01 vs. Control; # P < 0.05, ## P < 0.01 vs. TNF-α (+) Simvastain (−) group. Bar = 30 μm.

    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Techniques: Immunofluorescence, Expressing, Staining

    Differential expression of Cx37, Cx40 and Cx43 mRNAs in HUVECs across groups. Cx37 and Cx40 had a 2-fold downregulation, whereas Cx43 was upregulated relative to the control group. Cx37 and Cx40 were significantly upregulated in HUVECs treated with TNF-α in combination with simvastatin relative to those treated with TNF-α alone. Cx43 was significantly downregulated. Data presented are means ± SEM, n = 6, ** P < 0.01 versus only TNF-α treatment group.

    Journal: Animal Cells and Systems

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α

    doi: 10.1080/19768354.2021.2023037

    Figure Lengend Snippet: Differential expression of Cx37, Cx40 and Cx43 mRNAs in HUVECs across groups. Cx37 and Cx40 had a 2-fold downregulation, whereas Cx43 was upregulated relative to the control group. Cx37 and Cx40 were significantly upregulated in HUVECs treated with TNF-α in combination with simvastatin relative to those treated with TNF-α alone. Cx43 was significantly downregulated. Data presented are means ± SEM, n = 6, ** P < 0.01 versus only TNF-α treatment group.

    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Techniques: Expressing

    Cx37, Cx40 and Cx43 proteins were differentially expressed in HUVECs in different treatment groups. A: Western blots. 1: Control, 2: TNF-α (+)/Simvastatin (−), 3: TNF-α (+)/Simvastatin (+). B: qRT-PCR results showing relative transcript levels. Data presented are means ± SEM, n = 6, ** P < 0.01 versus only TNF-α treatment group.

    Journal: Animal Cells and Systems

    Article Title: Beneficial effect of simvastatin on human umbilical vein endothelial cells gap junctions induced by TNF-α

    doi: 10.1080/19768354.2021.2023037

    Figure Lengend Snippet: Cx37, Cx40 and Cx43 proteins were differentially expressed in HUVECs in different treatment groups. A: Western blots. 1: Control, 2: TNF-α (+)/Simvastatin (−), 3: TNF-α (+)/Simvastatin (+). B: qRT-PCR results showing relative transcript levels. Data presented are means ± SEM, n = 6, ** P < 0.01 versus only TNF-α treatment group.

    Article Snippet: The membranes were blocked with with blocking buffer (PBS containing 3%BSA and 0.1% sodium azide), for the reason that the molecular weight of Cx isoforms (37, 40, 43) and β-actin (42KD) are very close, then incubated for 1 h at room temperature with the following primary antibodies: rabbit polyclonal anti-Cx37 antibody (rabbit polyclonal Cx37 antibody raised against human; Cat. No. SAB4501180, Sigma-Aldrich), anti-Cx40 antibody (rabbit polyclonal Cx40 antibody raised against human; Cat. No. SAB1304973, Sigma-Aldrich) and anti-Cx43 antibody (rabbit polyclonal Cx43 antibody raised against human; Cat. No. C6219, Sigma-Aldrich) (1:1000) and anti-β actin antibody (rabbit polyclonal antibody raised against human; Cat. No. SAB5500001, Sigma-Aldrich) (1:1000).

    Techniques: Western Blot, Quantitative RT-PCR